Resolve example: Initiation factor 5a
(See: Peat, T. S., Newman, J., Waldo, G. S., Berendzen, J. & Terwilliger, T. C. (1998) "Structure of translation initiation factor 5A from Pyrobaculum aerophilum at 1.75 A resolution" Structure 15, 1207-1214.
An application of resolve
- MAD dataset on initiation factor 5A
- Only 1 of 3 selenium atoms in 147 residues used in phasing (just for this test example)
- Solvent content was 60%
- Resolution of 2 A, space group I4
- Initial correlation of experimental map with one based on refined model was 0.37
- Correlation after resolve was 0.79
- Correlation after conventional solvent flattening and histogram matching (using dm) was 0.65
Initial MAD map (CC=0.37)
Resolve map (CC=0.79)
Conventional solvent flattening & histogram matching (CC=0.65)