ribbons-demo -n trna

Data Files:

A F-MET t-RNA modelled by Prabhakaran was used.

A secondary structure assignment was made automatically:
pdb-nuc-ss trna.pdb trna.ss

This file was then edited by hand to add a code for the `domains' of the tRNA molecule.

The *.ribbons file consists of only one filename:
ls trna.ss > trna.ribbons

This file was then edited to add a special color file, `nucleic.color'.

The *.coords file consists of only one filename:
ls trna.pdb > trna.coords

This file was then edited to add the special `nucleic' key.

The *.model file file was created, then edited to change the menu name:
pdb-model trna.pdb trna.model

The model was displayed at this stage to color and select the special `base-attachment' bonds:
ribbons
( display the ribbon, open Ribbon Panel. set Sequence Color = `dom', set Print Select = `base-A', Print File => trna_ba0.rcyl Exit ribbons )

This cylinder file was renamed:
mv trna_ba0.rcyl trna_ba.rcyl

Created white bonds to outline the bases:
pdb-range-sph -b 0 -h trna.pdb trna.bb
( range 1,77; color 7)
sph-bond trna.bb trna_base.cyl

The *.bonds file consists of two filenames:
ls trna_ba.rcyl > trna.bonds
ls trna_base.cyl >> trna.bonds

Create the solid aromatic ring polygons:
pdb-nuc-ring trna.pdb trna-ring.tri

Create the *.polys file from this single set:
ls trna-ring.tri > trna.polys

The complete nucleic acid model is now ready for display.

Image File: $RIBBONS_HOME/rgb/trna.rgb

The model was displayed with default settings on the Ribbon Panel for everything except:
Sequence Color = `dom'
Ribbon Style = `Square'
Ribbon Texture = `Dipole'
Sheet Width = `6.0'
Helix Width = `0.3'
Ribbon Sample = `13'

Adjusted the bond radii of the `trna_base.bond' list to 0.5. The image was saved from the Geom Panel.


Ribbons User Manual / UAB-CMC / carson@cmc.uab.edu